CRISPR/CAS9 MUTAGENESIS REVEALS AN ESSENTIAL ROLE OF PI4KB IN PROMOTING GROWTH AND RESISTING HEMORRHAGIC DISEASE CAUSED BY GCRV-II INFECTION IN JUVENILE GRASS CARP

Few studies have reported obtaining grass carp resistant to hemorrhagic disease via gene editing in commercial fish.Here, we demonstrate that the expression and activity of grass carp PI4KB (gcPI4KB) are vital for GCRV-I and GCRV-II replication.Given the obvious cytopathic effect (CPE) in the present available cell lines is only caused by GCRV-I, b

This cross-sectional study aimed to assess the performances of a rapid diagnostic test (RDT)—the AllTest Malaria p.f./p.v., microscopy, and nested polymerase CHEESE BOARD chain reaction (PCR) for diagnosing Plasmodium falciparum malaria in 400 febrile patients from a low-transmission region (Jazan) in southwestern Saudi Arabia.Diagnostic performa

Global risk scores use individual level information on non-modifiable risk factors (such as age, sex, ethnicity and family history) and modifiable risk factors (such as smoking status and blood pressure) to predict an individual’s absolute risk of an adverse event over a specified period of time in the future.Cardiovascular risk scores have two m

Nearly 40% of the world population use biomass as their primary energy source, especially for cooking; but traditional and inefficient three-stone fires cause serious   health issues and their high fuel consumption leads to deforestation problems.Ethiopian cuisine is mainly based on vegetable and meat dishes in the form of a thick stew, served